Development of a simple and rapid method for typing population origins of the honey bee (Apis cerana) in Thailand
SC Imjongjirak, L Ngewsra, C Pramual, S Insuan, K Pala-or, S Klinbunga and S Sittipraneed
17 to 20
A simple and reliable method for determining the population origins of the honey bee (Apis cerana) in Thailand was developed based on restriction endonuclease analysis (REA) of genomic DNA with Hae III. Three profiles, A (9.6, 6.5 and 4.1 kb), B (12.0, 6.0 and 4.1 kb) and C (8.4, 5.7, 4.1 and 2.6 kb), were observed. Pattern A was found only in specimens originating from the northern Thailand population (north, north-east and the central region) whereas pattern B was observed in all bees from peninsular Thailand and 35% of those from Samui Island. Pattern C was not found in the mainland populations but restricted to bees from the Samui population (65%). Results indicated that REA- Hae III produced comparable results to PCR-RFLP of mtDNA gene segments, but were less tedious and more cost effective to perform.
honey bees, Apis cerana, DNA, restriction endonuclease analysis, REA, population typing, population origins, Thailand